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Final concentration is 0
CSM is also a basis for many “Drop-Out” mixtures to select for auxotrophic requirements and transformants
2H2O = 372
The antibiotic interferes with the translation step of polypeptide synthesis of prokaryotes and eukaryotes
2 at 25°C Suspend 25 gram powdered medium in 1 litre distilled water
YEP Broth Size:500g Final concentration is 0YEP Medium is based upon YPD but is without dextrose and can be used as a base for making YPD media with an alternate carbon source. To make YEPG Medium add 38 ml glycerol per litre YEP medium. Glycerol is a non fermentable carbon source. Respiratory deficient and or pet mutants will not grow on YPEG. Formula g l Yeast extract 10 Peptone 20 Suspend 30 gram powdered medium in 1 litre distilled water Store dry at room temperature.
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